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Sysmex TBNK test system

Lymphocyte subset testing

Flow cytometric immunophenotyping to evaluate the percentages and absolute counts of lymphocyte subsets is a well-established test that is performed in a variety of settings. It is primarily used for monitoring CD3+CD4+ T cells in human immunodeficiency virus (HIV)-infected individuals, and also in the evaluation of other forms of immune deficiency and autoimmune diseases [1-4].

Unique features of the Sysmex TBNK test system

The Sysmex TBNK test system on the XF-1600 platform provides accurate counts directly from the flow cytometer, without the need for counting beads. This is made possible by the volumetric count capability of the XF-1600 based on a calibrated syringe-driven acquisition of exact sample volume for accurate white blood cell (WBC) count – technology that is inherited from Sysmex’s XN-Series haematology analysers.

The resulting absolute counts are metrologically traceable to an internationally recognised reference method for white blood cells (WBC) [5].

More details

Your benefits in routine

  • The standardisation and convenience afforded by a preconfigured solution:
  • The assurance of reliability through built-in quality control procedures
  • Automation options - automated sample acquisition on the XF-1600 flow cytometer and optional automated sample preparation on the PS-10 sample preparation system
  • Streamlined workflow – all steps from test request to reporting are streamlined by Sysmex digital solutions

 

1) Warnatz, K. and Schlesier, M. Flow cytometric phenotyping of common variable immunodeficiency. Cytometry, 2008;74B: 261-271. https://doi.org/10.1002/cyto.b.20432
2) World Health Organisation. Consolidated guidelines on HIV prevention, testing, treatment, service delivery and monitoring: recommendations for a public health approach. 2021. ISBN: 978-92-4-003159-3
3) McCusker C, Upton J, Warrington R. Primary immunodeficiency. Allergy Asthma Clin Immunol. 2018;14(Suppl 2):141-152. https://doi.org/10.1186/s13223-018-0290-5
4) Rabson A. Enumeration of T-cells subsets in patients with HIV infection. AIDS Clin Care. 1995 Jan;7(1):1-3. PMID: 11362161.https://pubmed.ncbi.nlm.nih.gov/11362161/
5) Ward RY, Stevens M, Bashir S. Metrological traceability in flow cytometry? Evaluation of a new volumetric method for lymphocyte subsets. Int J Lab Hematol. 2023;1‐7. doi:10.1111/ijlh.1421 https://doi.org/10.1111/ijlh.14219

Technical specifications

Results

IVD parameters Relative percentages** and absolute counts* of:
  • CD3+ (CD45+ & CD3+)
  • CD4+ (CD45+ & CD3+ & CD4+ & CD8-)
  • CD8+ (CD45+ & CD3+ & CD4- & CD8+)
  • CD16/56+ (CD45+ & CD3- & CD16/56+ & CD19-)
  • CD19+ (CD45+ & CD3- & CD16/56- & CD19+)
Research Parameters
Analysis results for these research parameters must not be used for diagnosis of patients

Absolute counts* of:

  • WBC (CD45+)
  • Lymphocytes
Absolute counts* and Percentages (%)**
  • CD3- (CD45 & CD3-)
  • CD3+CD4+CD8+

Sums and ratios

  • T-Sum (%Gated for CD3+ minus %Gated for the following: (CD3+CD4+CD8-), (CD3+CD4+CD8+), CD3+CD4-CD8+)
  • Lymphsum
  • CD4/8 ratio

*Number of cells per μL

**Ratio to lymphocytes (based on a lymphocyte gate)

Contact us
You have a question and would like to get in touch with our experts? We look forward to receiving your enquiry.

Sysmex Europe SE

Bornbarch 1

22848 Norderstedt

Germany

+49 (40) 527 26 0

+49 (40) 527 26 100

Training

TBNK basic online training

After this course you will be able to perform and interpret the TBNK application using the XF-1600. This training is intended for operators to run the TBNK application.

Enroll here

Documents

Product documents

Regulatory documents

Regulatory documents, such as Instructions for Use, can be accessed with a valid My Sysmex login:

Go to My Sysmex

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XF-1600

XF-1600

Clinical flow cytometer for immunophenotyping

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