Expert-led, evidence-based and future-proof content
Unparalleled uniformity and depth of coverage for difficult to sequence genes, e.g. CEBPA, NPM1 and FLT3-ITDs
Rapid, streamlined workflow with Universal NGS Complete Workflow Solution
Complimentary data analysis software with Interpret NGS Analysis Software

The SureSeq^TM Myeloid MRD panel delivers

The SureSeq^TM Myeloid MRD Panel has been designed in collaboration with leading cancer experts and in accordance with the European LeukemiaNet (ELN) recommendations [1,2] to offer a single, cost-effective NGS assay to investigate MRD in AML samples. The panel content incorporates key genes for assessing AML, including genes implicated in MPN and MDS, to investigate secondary AML progression.

In addition, genes related to research on potential drug response are included for truly comprehensive and informative sample analysis.

Utilising SureSeq’s intelligent panel design capabilities, the Myeloid MRD Panel surpasses the typical sensitivities offered by alternative NGS panels to accurately detect SNVs, indels and internal tandem duplications (ITDs) down to 0.05% VAF.

Gene targets

Gene	*Exons*
CSF3R	Exons 13–17
MPL	Exon 10
SF3B1	Exons 13–16
IDH1	Exon 4
KIT	Exons 2, 8–11, 13 and 17
NPM1	Exon 11
JAK2	Exons 12 and 14
FLT3	Exons 13-15 et 20
IDH2	Exons 4 and 5
TP53	Exons 2–11 (inc. NM_001276695:ex10, NM_001276696:ex10)
CALR	Exon 9
Exon 9	Exons 4–8
CEBPA	Exon 1

More details

Figure 1: CALR Exon 9 (hg19 chr19:13054527-13055304). Depth of coverage per base (grey). Targeted region (green). Gene coding region as defined by RefSeq (blue). GC percentage (red).

Figure 2: IGV plot showing high uniformity of coverage of all targeted regions in the panel, including NPM1 exon 11.

Figure 3: FLT3-ITDs of various sizes and even regions containing multiple ITDs can be confidently detected. ITD sizes are [A] 174 bp, [B] 225 bp, [C] 195 bp with additional 6 bp, [D] 120 bp, [E9 168 bp with additional 69 bp.

Haematological NGS panels

View all NGS library preparation kits & reagents

References

[1] European LeukaemiaNET. Available at: www.leukemia-net.org.

[2] Döhner et al Blood. 2022 ;140(12):1345-1377

Disclaimer

SureSeq^TM: For Research Use Only, not for use in diagnostic procedures. Product availability may vary from country to country and is subject to varying regulatory requirements. Contact your local representatives for availability.

Manufacturer and Trademarks: SureSeq^TM(Oxford Gene Technology IP Limited)

Technical specifications

Technical information

Feature	Specification
Number of targets	45 hotspot exons from 13 genes
Panel size	11.2 kb
Mean target coverage	Up to 20,000x
Limit of detection SNVs, indels, ITDs	0.05%	0.1%
DNA input recommended	2 x 200 ng	200 ng
	Samples per run
NextSeq 500 High Output	16	24
NextSeq 2000 P3	48	72
NovaSeq® SP	32	48
NovaSeq S1	64	96

Detection of SNVs, indels and an ITD, with expected frequency ranges of 0.1%-0.05%. SNVs are filtered to remove unique molecular identifiers (UMI) with a read family size of 1. A Myeloid Reference DNA Standard (Horizon Discovery) sequenced on a Illumina NextSeq^® 500.

	Gene	Variant	Coordinate	0.1% VAF	0.1% VAF	0.05% VAF	0.05% VAF	Neg. ctrl	Neg. ctrl
				Read depth	Observed VAF	Read depth	Observed VAF	Read depth	Observed VAF
SNV	SF3B1	c.2219G>A	chr2:197401989	7426	0.13	14282	0.03	15076	0
	IDH1	c.394C>T	chr2:208248389	6743	0.09	15572	0.06	15734	0
	JAK2	c.1849G>T	chr9:5073770	10408	0.12	22348	0.08	22065	0
	FLT3	c.2503G>T	chr13:28018505	9747	0.11	24098	0.04	23942	0
	IDH2	c.515G>A	chr15:90088606	7294	0.08	16554	0.04	18875	0
	TP53	c.722C>T	chr17:7674241	5166	0.12	12609	0.03	13769	0
Indel	NPM1	c.860_863dup	chr5:171410539	8164	0.11	18630	0.07	17630	0
	JAK2	c.1611_1616del	chr9:5070021	12270	0.03	28197	0.02	25411	0
ITD	FLT3	ITD300	chr13:28033909	6045	≥ 0.1	12265	≥ 0.05	10473	0

Ordering information

Product	Contents	Cat. No.
SureSeq Myeloid MRD Complete NGS Workflow Solution V2 (48)	Enrichment baits sufficient for 12 hybridisation reactions. Bundle of 1 x Universal Library Preparation Kit (96) containing PCR primers and enzymes. 1 x Universal Hybridisation & Wash Kit V2 (96). 1 x Pre-PCR Universal Bead Kit (96). 1 x Post-PCR Universal Bead Kit (96). 1 x Universal Index Adapter Kit (96). Interpret NGS Analysis Software	780126-48
SureSeq Myeloid MRD Panel (48)	Enrichment baits sufficient for 12 hybridisation reactions. Interpret NGS Analysis Software	770026-48
Universal NGS Workflow Solution V2 (96)	Bundle of 1 x Universal Library Preparation Kit (96) containing PCR primers and enzymes, 1 x Universal Hybridisation & Wash Kit V2 (96). 1 x Pre-PCR Universal Bead Kit (96). 1 x Post-PCR Universal Bead Kit (96). 1 x Universal Index Adapter Kit (96)	770510-96