gain a more complete understanding of CLL progression: Accurately detect low-frequency SNVs and indels in 16 key CLL-implicated genes, including TP53, BTK, PLCG2, BCL2 and NRAS.
enhanced CNV detection ranging from small copy number variations to full chromosome arms and trisomy 12: Profile your samples for CNVs in the five most commonly aberrant regions in CLL.
time savings: Replace multiple assays with a single NGS panel, increasing throughput and reducing turnaround time.
complimentary data analysis software: Analyse your data with Interpret NGS Analysis Software, a powerful and easy-to-use analysis solution for accurate identification of all variants and CNVs.

Chronic lymphocytic leukaemia (CLL) is the most common type of leukaemia in adults. A wide variety of chromosomal abnormalities are associated with CLL, ranging from single nucleotide variants (SNVs) and insertions/deletions (indels) to large copy number variations (CNVs), including trisomies¹.

The SureSeq^TM CLL + CNV V3 Panel has been designed in collaboration with recognised cancer experts to detect 16 key genes and five chromosomal regions implicated in CLL progression (Table 1). The SureSeq CLL + CNV V3 Panel alleviates the burden of running multiple assays and streamlines your CLL research to deliver a comprehensive genomic profile for each CLL sample using a single workflow.

Table 1: Gene targets

Genes	ATM, BCL2, BIRC3, BRAF, BTK, CXCR4, KRAS, MYB, MYD88, NOTCH1, NRAS, PLCG2, SAMHD1, SF3B1, TP53 and XPO1
CNVs	del17p (covering TP53), del11q (covering ATM), del13q (covering RB1/DLEU2/DLEU7), del6q (6q23.2- 6q23.3 covering MYB) and Trisomy 12. Reference DNA is included to provide a baseline for CNV calling.

More details

Figure 1: Illustration of the excellent uniformity and high depth of coverage, allowing confident detection of [A] a SF3B1 exon 15 hotspot variant Lys700Glu with 4.8% allele frequency and [B] a TP53 exon 4 frameshift deletion (TP53 c.124del) with frequency 38.9%.

NGS

Array

Figure 2: 42.7 Mb deletion of 11q covering ATM.

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Array

Figure 3: 0.6 Mb biallelic loss called within a larger ~1 Mb single allele deletion in the region covering DLEU2/DLEU1/DLEU7 on chromosome 13q.

Figure 4: Following analysis, all variants and CNVs are visualised for easy interpretation in OGT’s Interpret NGS Analysis Software. In this example, a trisomy 12 is detected, showing a reliable gain call across the whole chromosome.

Haematological NGS panels

View all NGS library preparation kits & reagents

References

[1] Alaggio et al. Leukemia 2022 36(7):1720-1748

Disclaimer

SureSeq^TM: For Research Use Only, not for use in diagnostic procedures. Product availability may vary from country to country and is subject to varying regulatory requirements. Contact your local representatives for availability.

Manufacturer and Trademarks: SureSeq^TM (Oxford Gene Technology IP Limited)

Technical specifications

Technical information

Feature	Specification
Number of genes	16
Panel size	142 kb
DNA input recommended	>500ng high-quality DNA
Gene list	ATM, BCL2, BIRC3, BRAF, BTK, CXCR4, KRAS, MYB, MYD88, NOTCH1, NRAS, PLCG2, SAMHD1, SF3B1, TP53 and XPO1
CNV list	del17p (covering TP53), del11q (covering ATM), del13q (covering RB1/DLEU2/DLEU7), del6q (6q23.2-6q23.3 covering MYB) and Trisomy 12
Sample tracking	SRY + 24 SNP profiling panel
Limit of detection	SNVs/indels: capable of VAF of 1 - 2.5% within the 16 genes. CNVs: capable of detecting CNVs down to 20% tumour content
SureSeq Reference Human DNA	SureSeq Reference Female DNA, SureSeq Reference Male DNA

Ordering information

Product	Contents	Cat. No.
SureSeq CLL + CNV V3 Complete NGS Workflow Solution V2 (24)	Enrichment baits sufficient for 3 x 8-sample pools. Bundle of 1 x Universal Library Preparation Kit (24), containing PCR primers and enzymes. 1 x Universal Hybridisation & Wash Kit V2 (24). 1 x Pre-PCR Universal Bead Kit (24). 1 x Post-PCR Universal Bead Kit (24). 1 x Universal Index Adapter Kit (24). SureSeq Reference Human DNA. Interpret NGS Analysis Software	780106-24
SureSeq CLL + CNV V3 Complete NGS Workflow Solution V2 (96)	Enrichment baits sufficient for 12 x 8-sample pools. Bundle of 1 x Universal Library Preparation Kit (96), containing PCR primers and enzymes. 1 x Universal Hybridisation & Wash Kit V2 (96). 1 x Pre-PCR Universal Bead Kit (96). 1 x Post-PCR Universal Bead Kit (96). 1 x Universal Index Adapter Kit (96). SureSeq Reference Human DNA. Interpret NGS Analysis Software	780106-96
SureSeq CLL + CNV V3 Panel (24)	Enrichment baits sufficient for 3 x 8-sample pools. SureSeq Reference Human DNA. Interpret NGS Analysis Software	770040-24
SureSeq CLL + CNV V3 Panel (96)	Enrichment baits sufficient for 12 x 8-sample pools. SureSeq Reference Human DNA. Interpret NGS Analysis Software	770040-96
Universal NGS Workflow Solution V2 (24)	Bundle of 1 x Universal Library Preparation Kit (24), containing PCR primers and enzymes. 1 x Universal Hybridisation & Wash Kit V2 (24). 1 x Pre-PCR Universal Bead Kit (24). 1 x Post-PCR Universal Bead Kit (24). 1 x Universal Index Adapter Kit (24)	770510-24
Universal NGS Workflow Solution V2 (96)	Bundle of 1 x Universal Library Preparation Kit (96), containing PCR primers and enzymes. 1 x Universal Hybridisation & Wash Kit V2 (96). 1 x Pre-PCR Universal Bead Kit (96). 1 x Post-PCR Universal Bead Kit (96). 1 x Universal Index Adapter Kit (96)	770510-96